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Browsing by Author "Eman Saleh Alhasani"

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    Development of New Drug against Multidrug Resistance Candida auris by Mining Genome from Marine Bacteria
    (2023) Eman Saleh Alhasani; Noor B Almandil
    The most common multi-drug-resistant fungal infection in the Arabian Peninsula is Candida auris, which is responsible for high mortality. Identifying novel drugs is in high demand in this scenario. Microbes in marine environments are genetically adapted to survive in varying conditions like extreme temperatures, salinity, pH, and extra stress features by synthesizing different bioactive metabolites. These microbial bioactive novel secondary metabolites are highpotential compounds that can be utilized as anti-Candida molecules. Microorganisms in the marine environment can produce diverse ranges of secondary metabolites (SM), which are also known as natural products. Finding and identifying novel drugs from synthetic bacterial compounds or natural materials is a promising strategy due to the scarcity of antifungal medications and the evolution of drug resistance in C. auris. Natural compounds have many benefits over synthetic substances, including structural variety and comparatively low toxicity. Next-generation sequence-based studies on anti-Candida auris bacterial organisms for discovering novel drug candidates are scanty. Hence, this study is proposed to identify biosynthetic gene clusters from marine bacteria using next-generation sequencing for discovering novel drug compounds against MDR C. auris. More than 68 isolates have been isolated from various marine environments using standard techniques. All these isolates were tested against the multi-drug-resistant C. auris. Bacterial strain ESH58 isolated from a fish liver sample showed the highest and most constant activity against C. auris. Cell membrane disruption was observed in C. auris by the defused metabolites of ESH58 bacteria using SEM. An experimental in vitro assessment of the toxicity revealed no cell cytotoxicity of ESH58 against HFF-1 cells. The native bacterial strain ESH58 with a biosynthetic gene cluster of potential natural anti-C. auris compounds with no pathogenic protein-coding genes highlights the significance of marine bacteria in the fight against Candida auris. After the in vivo studies, drug targets will be taken for the pre-clinical studies in association with pharmaceutical companies in the future.