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معهد البحوث والاستشارات الطبية | Institute for Research and Medical Consultations

Permanent URI for this collectionhttps://repository.iau.edu.sa/handle/123456789/336

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Browsing معهد البحوث والاستشارات الطبية | Institute for Research and Medical Consultations by Subject "Cytotoxicity"

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    Study the toxicity and biocompatibility aspects of Zinc Phosphate [Zn3(PO4)2] nanoparticles in Mice and Rats and their effect on body functions.
    (2023) Noha Mubarak Bin Mahfooz; Abdelgadir Musa Homeida
    Nanomaterials (NMs), substances that have internal or surface structure dimension that is at the nanoscale (less than 100 nm) have been employed throughout biomedical research studies The objective of this study was to synthesize Zinc Phosphate Nanoparticles (ZnP NPs) Zn3(PO4)2, analyse the chemical characteristic, structure and morphology, study the cytotoxicity and haemolytic effect in-vitro and to investigate the acute toxic potential in adult male rats and mice. In this study, a formulation of phosphate-based zinc nanoparticles was synthetized and tested. The formulation of zinc nanoparticles based on phosphates with irregular morphology were prepared. Transmission electron microscopy (TEM) was used to examine the morphology and size of Zn3(PO4)2The size average was about 43 nm as calculated by TEM image. It also shows that nanoparticle is asymmetrical in shape, semispherical and includes heterojunctions in-between. Scanning electron microscopy (SEM), demonstrates the surface of ZnP NPs which looks like fine flakes. The NPs produced in-vitro 20% haemolysis of rat red blood cells a concentration of 1mg/mL. The MTT assay (cell viability) was performed in normal cell line of Human embryonic kidney (HEK 293). Zinc phosphate nanoparticles showed a non-toxic effect on HEK 293 cells when the concentration of NPs was lower than 1mg/ mL. However, the concentration of the nanoparticles is directly proportional with cell toxicity, higher the concentration, more the toxicity. The highest in-vitro inhibitory effect of our NPs was observed against viability of cells. After the successful in-vitro testing, the in vivo LD50 was determined in rats and mice. Values of LD50 acute toxicity of zinc phosphate nanomaterials were determined. According to Hodge and Sterner toxicity scale, the obtained value of LD 50 of zinc phosphate nanoparticles in rats is 2512 mg/Kg body weight which is of slightly toxic category. In mice the LD50 is 5000 mg/Kg body weight which is practically non-toxic. The clinical blood chemistry analyses showed no significant differences in serum metabolites or enzyme activities between the control group and the exposed rats dosed with 300mg/Kg body weight. However, enzyme activities, and concentration of blood urea nitrogen and uric acid were significantly increased in rats dosed with 2000 mg/Kg body weight. The results indicated that ZPNMs are nontoxic at a dose of 1mg/mL in-vitro and at a dose of 300mg/Kg body weight in-vivo in rats.