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الابحاث العلمية للطلاب |Students Research Articles

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يعرض هذا القسم الأبحاث المقبولة للنشر ضمن مبادرة نشر الأبحاث للطلبة في المجلات ذات التأثير المرتفع و التي اطلقتها عمادة البحث العلمي

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Browsing الابحاث العلمية للطلاب |Students Research Articles by Author "A. H. A. Alabdalall, F. A."

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    Degradation of 2,6-dicholorophenol by Trichoderma longibraciatum Isolated from an industrial Soil Sample in Dammam, Saudi Arabia
    (2022) Rawan Aldughaish; Ababutain, I. M.; Chakroun, H.; Inès Hammami; Hammami, I.; Ibtisam Mohammed Ababutain; Azzah Ibrahim Alghamdi; Albarrag, A. M.; Aldakeel, S. A.; Aldughaish, R.; Al Qurin, N.; ElKomy, H. M.
    2,6-Dichlorophenol (2,6-DCP) is an aromatic compound with industrial importance in making insecticides, herbicides, and other organic compounds. However, it poses serious health and ecological problems. Microbial degradation of 2,6-DCP has been widely applied due to its effectiveness and eco-friendly characteristics. In this study, Trichoderma longibraciatum was isolated from an industrial soil sample in Dammam, Saudi Arabia using the enrichment method of mineral salt's medium (MSM) amended with 2,6-DCP. Morphological and molecular identification (using the internal transcribed spacer rRNA gene sequencing) of the 2,6-DCP tolerating fungal isolate were charactraized. The fungal isolate has demonstrated a tolerance to 2,6-DCP up to 300 mg/L. Mycelial growth and fungal sporulation were reduced with increasing 2,6-DCP concentrations up to 96 h incubation period. However, after 168 h incubation period, the fungal isolate recorded maximum growth at all the tested 2,6-DCP concentrations up to 150 mg/L. Carboxy methyl cellulase production by tested fungus was decreased by increasing 2,6-DCP concentration up to 75 mg/L. The biodegradation pattern of 2,6-DCP in GM liquid medium using GC-mass analysis as well as the degradation pathway was presented. This study provides a promising fungal isolate that could be used in the bioremediation process for chlorinated phenols in soil.